OSTEOIMAGER Side Scanner

The BIOQUANT OSTEOIMAGER slide scanner is an inverted automated scanning microscope. It scans both histology sections and cell cultures. A motorized nosepiece allows it to scan with 4X, 10X, 20X, or 40X objectives. It scans in brightfield, multi-channel fluorescence, polarized light, or darkfield.

BIOQUANT SCAN

BIOQUANT SCAN is the control software for OSTEOIMAGER. BIOQUANT SCAN is used to specify objectives, control illumination sources, adjust focus, define scan areas, and export images.

Technical Specifications

Unified LIGHT PATH

OSTEOIMAGER uses a unified light path that eliminates the need to adjust the microscope when switching between brightfield and fluorescence imaging. Simply turn off one light and turn on the other.

Multi-modal CondenseR

To switch from brightfield to polarized light to darkfield simply rotate the condenser wheel to insert or remove the appropriate filter.

Optical Specifications

Fluorite Objectives 4X 0.13NA, 10X 0.25NA, 20X 0.5NA, 40X 0.75NA
Long Working Distance Condenser 25mm Working Distance, 0.55NA

Illumination Specifications

Transmitted Light
10,000 Hour White LED
Epi-fluorescent Light
10,000 Tunable LED Illuminator
Tripe BandFilter Cube
DAPI/Calcein/Alizarin
Polarized Light
Linear Polarization, All Objectives
Darkfield Light
4X and 10X Objectives

Specimen Handling

Multi-slide Holder Fixed Vertical Orientation, 4 Slides, 25mm x 75mm
Single-slide Holder 360° Rotation, 1 Slide, 25 x 75mm or 50 x 75mm
Well Plate Holder 1 plate, Standard 84mm x 127mm

Scanning Specifications

Imaging Camera Jenoptik Prokyon - 2.3 / 20 Megapixel, Color, 60fps
Focus Camera Watec 902H3 - 0.3 Megapixel, Monochrome, 30fps
Maximum Scan File Size 4GB Uncompressed
Scan File Formats Calibrated BIF, Uncalibrated TIF
Scan Area 4X over 2500 mm2 (1.4 microns per pixel)
Scan Area 10X over 400 mm2 (0.56 microns per pixel)
Scan Area 20X over 100 mm2 (0.28 microns per pixel)
Scan Area 40X over 25 mm2 (0.14 microns per pixel)
Maximum / Auto Focus Scan Rate 1.5s per field / 5s per field

Brightfield Imaging

Example of Background Correction

Live Correction dynamically corrects the live image for the uneven lighting.

Uncorrected Brightfield Background.

Live Background Correction Applied.

Examples of non-demineralized histology

Osteoblasts and Adipocytes

Osteoclasts

Live Multicolor Fluorescence Mixing

Examples of live multicolor fluorescence mixing with reduced excitation and increased excitation. 20x objective. Calcein and alizarin red labels.

Reduced Excitation

Increased Excitation

Reduced Excitation

Increased Excitation

Reduced Excitation

Increased Excitation

Live Muliticolor Fluorescence Examples

20X objective. 0.4 microns per pixel. Trabecular bone. Calcein and alizarin red labels. Blue autofluorescence in mineralized bone. Hardware brightness adjustment and simultaneous viewing. Live black background correction is also applied in hardware. No software post processing required.