OSTEOIMAGER Side Scanner

The BIOQUANT OSTEOIMAGER slide scanner is an inverted automated scanning microscope. It scans both histology sections and cell cultures. A motorized nosepiece allows it to scan with 4X, 10X, 20X, or 40X objectives. It scans in brightfield, multi-channel fluorescence, polarized light, or darkfield.

Supports BIOQUANT SCAN

BIOQUANT SCAN is the control software for the OSTEOIMAGER. BIOQUANT SCAN is an extension to the BIOQUANT OSTEO or BIOQUANT Life Science analysis software. BIOQUANT SCAN is used to specify objectives, control illumination sources, adjust focus, define scan areas, and export images.

Unified Transmitted / Reflected Light Path

The OSTEOIMAGER slide scanners is designed around a uniform light path that eliminates the need to adjust the microscope when switching between brightfield and fluorescence imaging. Simply turn off one light and turn on the other.

Multi-modal Condenser Wheel

To switch from brightfield to polarized light to darkfield simply rotate the condenser wheel to insert or remove the appropriate filter.

Technical Specifications

Optical Specifications

  • Olympus UIS2 Fluorite Objectives 4X 0.13NA, 10X 0.25NA, 20X 0.5NA, 40X 0.75NA

  • Olympus IX2-LWUCD Condenser 25mm Working Distance, 0.55NA

Illumination Specifications

  • Transmitted Light 10,000 Hour White LED

  • Epi-fluorescent Light 10,000 Hour Broad Spectrum LED Illuminator

  • Fluorescent Filter Cube Triple Band ET DAPI/Calcein/Alizarin Red (Changeable) 

  • Polarized Light Linear Polarization, All Objectives

  • Darkfield Light 4X and 10X Objectives

Sample Handling Specifications

  • XY Motorized Stage Applied Scientific Instrumentation Model MS2000-FT

  • Z Motorized Focus Applied Scientific Instrumentation Model LS50

  • Multi-slide Holder Fixed Vertical Orientation, 4 Slides, 25mm x 75mm

  • Single-slide Holder 360° Rotation, 1 Slide, 25 x 75mm or 50 x 75mm

  • Well Plate Holder 1 plate, Standard 84mm x 127mm

Imaging Specifications

  • Imaging Camera Jenoptik Prokyon - 2.3 / 20 Megapixel, Color, 60fps

  • Focus Camera Watec 902H3 - 0.3 Megapixel, Monochrome, 30fps

  • Maximum Scan File Size 4GB Uncompressed

  • Scan File Formats Calibrated BIF, Uncalibrated TIF

  • Scan Area 4X over 2500 mm2 (1.4 microns per pixel)

  • Scan Area 10X over 400 mm2 (0.56 microns per pixel)

  • Scan Area 20X over 100 mm2 (0.28 microns per pixel)

  • Scan Area 40X over 25 mm2 (0.14 microns per pixel)

  • Maximum / Auto Focus Scan Rate 1.5s per field / 5s per field

Imaging Overview

Brightfield Imaging

Example of Background Correction

Live Correction dynamically corrects the live image for the uneven lighting.

Examples of non-demineralized histology

Live Multicolor Fluorescence Mixing

Examples of live multicolor fluorescence mixing with reduced excitation and increased excitation. 20x objective. Calcein and alizarin red labels.

Live Muliticolor Fluorescence Examples

20X objective. 0.4 microns per pixel. Trabecular bone. Calcein and alizarin red labels. Blue autofluorescence in mineralized bone. Hardware brightness adjustment and simultaneous viewing. Live black background correction is also applied in hardware. No software post processing required.

Polarization and Darkfield

Examples of the same field in brightfield, linear polarized illumination, and darkfield illumination.

Sample Scans

Check out sample scans in the Scan Gallery on the BIOQUANT SCAN Add-on page.

Download Data Sheet with Schematic Diagrams

The following data sheet has more information about the OSTEOIMAGER scanning microscope, including schematic diagrams.