Examples of non-demineralized histology. Osteoclasts, osteoblasts, adipocytes, osteocytes, osteoid.

Left column: reduced excitation. Right column: increased excitation. First two rows: individual label viewing. Last row: live dual label viewing. 20X objective. Calcein and alizarin red labels.

20X objective. 0.4 microns per pixel. Trabecular bone. Calcein and alizarin red labels. Blue autofluorescence in mineralized bone. Hardware brightness adjustment and simultaneous viewing. Live black background correction is also applied in hardware. No software post processing required.

Representative multi-channel fluorescence scan of distal mouse femur at 0.3 micron per pixel resolution. Simultaneous viewing of calcein and alizarin red labels for bone formation rate. No software image merging step required. Both labels are imaged simultaneously.

Representative trichrome scan of distal mouse femur at 0.3 micron per pixel resolution.

Top: Ground Cortical Bone Cross-Section
Middle: Same field-Darkfield Illumination
Bottom: Same field - Linear Polarized Illumination