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Automated Cell Counting
Using Feulgen staining to linearly stain DNA contained in cell nuclei, BIOQUANT calculates the genome size of a target species based on the genome size of a referent species.
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Axon Number and Caliber
In semi-thin sections with myelin specific staining, BIOQUANT automatically detects myelinated axons. Detection is based on color, shape, and size. Shape filters automatically exclude obliquely cut axons.
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Retinal Layer Thickness
In retinal cross-sections, a specialized tool analyzes the thickness of consecutive layers. Once the boundaries between layers are traced, perpendicular thickness is measured automatically at regularly spaced intervals along the surface.
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Blood Vessel Stenosis
Using BIOQUANTs Editing Tools, the histologist defines the EEL boundaries. Once defined, BIOQUANT will use the defined threshold to automatically measure the EEL and IEL.
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Cell Proliferation Analysis
Using the DAB chromagen, BIOQUANT identifies PCNA+ chondrocytes. Size and shape filters exclude non-specific staining. Using the counterstain, BIOQUANT identifies the other chondrocytes in the referent area.
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Cellular mRNA Analysis
S35 radiolabeling and silver grain emulsion dipping identify mRNA signals in tissue sections. Using either standardized circular regions or free-drawn boundaries, BIOQUANT can analyze the degree of grain labeling in a cell population.
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Cell Mapping and Analysis
Using automated tracing algorithms, BIOQUANT measures cross-sectional area in serially sectioned volumes of tissue.
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Immunohistochemistry
Using a consistent "expression threshold," BIOQUANT rapidly quantifies the area of protein expression in specific anatomical regions.
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