Histology Services

Resin Embedding

The embedding of fixed, non-decalcified bone samples in methyl methacrylate for subsequent thin section microtomy or ground sectioning.

Decalcification and Paraffin Embedding

The decalcification of bone samples in EDTA so that they can be embedded in paraffin for sectioning.

Thin Section Microtomy

Resin embedded sections cut at the microtome between 5 and 10 microns thick. Commonly used in skeletal phenotyping in the distal femur, proximal tibia, or vertebra. Commonly used in the histomorphometry of human iliac crest biopsy.

Ground Sections

Resin embedded ground sections of thickness between 100 and 50 microns. Commonly used for cortical bone cross-sections and sectioning tissue with metal implants.

Staining in Non-Decalcified Bone Sections

Trichrome StaininG

For the visualization of mineralized bone, osteoid, osteoblasts, osteoclasts, osteocytes, and adipocyte.

Enzymatic TRAP Staining

For the visualization of TRAP positive cells like osteoclasts.

Toluidine Blue Staining

For the visualization of osteoid seams and osteoblasts.

Von Koss Staining

For the visualization of mineralized bone.

Immunohistochemistry

For the visualization of protein concentration and distribution.

Custom Staining

Our full-service lab can handle most staining protocols. Contact to discuss your specific requirements.